A study published in the journal of Environmental Toxicology and Chemistry revealed that glyphosate, the main ingredient found in the herbicide Roundup, was found in over 75% of air and rain samples tested in Mississippi in 2007.
The study is disconcerting as it reveals that involuntary exposure to the herbicide is worse than previously thought. Other reports confirm that exposure is not confined to one region but occurs on a global level. It has been calculated that about 0.7% of the glyphosate applied in agricultural areas is removed from the air in rain.
Glyphosate was first synthesized in Switzerland in 1950, then developed as a herbicide by Monsanto in 1970. The global production capacity is about 1.1 million tonnes, with China’s capacity alone being 835,900 tonnes.
According to the report, thirty-seven compounds were detected in the air or rain samples in 2007; 20 of these were present in both air and rain. Glyphosate was the predominant new herbicide detected in both air (86%) and rain (77%) in 2007, but was not measured in 1995.
Seven compounds in 1995 and five in 2007 were detected in ≥50% of both air and rain samples. Atrazine, metolachlor, and propanil were detected in ≥50% of the air and rain samples in both years.
Glyphosate residues have been found in soil where genetically modified (GM) soy is grown in Argentina, at levels up to 4.45 mg/L, and have been detected in surface waters where GM soy is grown in Argentina, at levels up to 0.56 mg/L and in sediment at 1.85 mg/L.
A one-year monitoring project of three wastewater treatment plants and one composting unit in France detected glyphosate and AMPA in all samples of sludge. Glyphosate and AMPA were found in tributaries of the river Ruhr in Germany in the 1990s at up to 590 ng/L; in 54% of surface water samples in Norway in the late 1990s at up to 0.93 µg/l glyphosate and 0.2 µg/L AMPA; and in elevated levels in surface water, soil and sediment samples in Argentina as a result of GM soy cultivation.
Glyphosate was found in 17.5% and AMPA in 67.5% of samples from 10 wastewater treatment plants in the US; and, in Canada, 21% of samples had concentrations up to 41 µg/L for glyphosate and 30 µg/L for AMPA. Landfill leachate Glyphosate has been detected in landfill leachate in the UK.
Glyphosate was found in the groundwater in 11 different locations in Catalonia, Spain. It was detected in 41% of 140 samples, at concentrations as high as 2.5 µg/L, 25 times the legal limit for drinking water in the EU.
Detectable concentrations of glyphosate and AMPA were found in marine sediment at a number of sites in the Waitemata Harbour and Hauraki Gulf in New Zealand. The maximum glyphosate concentration detected was 1 ppm, while AMPA had a maximum level of 0.37 ppm.
Previous studies have documented the chronic and mutagenic impact of Roundup. Roundup kills embryonic kidney cells at levels 200-fold lower that those recommended for agricultural use. Four Roundup formulations caused cell death in human liver cell lines, at concentrations described as “far below those used in agriculture”, and also below those legally allowed as residues in GM food.
Glyphosate, at low doses (15-70 µM), kills human skin cells by inducing mitochondrial membrane potential disruption leading to oxidative stress. Deregulation of the cell death mechanism or an improper removal of damaged cells can lead to cancerous lesions, and “confirms the potential public health risk” of glyphosate.
Both glyphosate and Roundup caused DNA damage in human buccal (mouth) epithelial cells with short-term (20 mins) exposure to
concentrations corresponding to a 450-fold dilution of concentrations normally used in agriculture. Even a 1,350-fold dilution of a
spraying solution of Roundup caused acute and genotoxic effects on human cells.
Doctors in Argentina have reported a dramatic upsurge in long-term effects in areas where genetically modified soy crops are aerial sprayed with glyphosate. They include cancer, infertility, pregnancy problems, birth defects, and respiratory diseases.
A report released by Argentina’s Chaco state government in April 2010 refers to a significant increase in cancer in La Leonesa, an agricultural town, including a 3-fold increase in childhood cancers, particularly leukaemia, lymphoma, and brain tumors, coinciding with the dramatic increase in transgenic crops such as soy which are heavily sprayed with glyphosate.
Birth defects in the Argentinean state of Chaco, where GM soy and rice crops are heavily sprayed with glyphosate, increased nearly fourfold over the years 2000 to 2009, according to a report released by the Chaco state government. Several cases of malformations together with repeated spontaneous abortions were detected in the village of Ituzaingó [Cordoba], which is surrounded by GMO-based agriculture”.
Another study showing higher incidences of spina bifida, microtia, cleft lip with cleft palate, polycystic kidney, postaxial polydactyly and Down’s syndrome in the intensive GM soy cultivation area in Cordoba. Similar birth defects were also experienced by 52 women in Paraguay, who were exposed during pregnancy to herbicides.
In 2009, Argentinean researchers lead by Professor Carrasco showed that 1/5000th of the concentration of commercial glyphosate disrupted the development of the craniofacial skeleton. The malformations occurred at 2.03 mg/kg glyphosate; residues have been found
in soybeans up to 17 mg/kg. The glyphosate caused marked alterations in cephalic and neural crest development and shortening of the anterior-posterior axis in tadpole embryos, resulting in deformities in the cranial cartilages at the tadpole stage. Other effects included shortening of the trunk, reduced head size, and eye defects; and the authors concluded their results were “compatible with the malformations observed in the offspring of women chronically exposed to glyphosate-based herbicides during pregnancy”.
Majewski MS, Coupe RH, Foreman WT, Capel PD Environ Toxicol Chem. 2014 Feb 19. doi: 10.1002/etc.2550. Pesticides in Mississippi air and rain: A comparison between 1995 and 2007.
Antoniou M, Brack P, Carrasco A, Fagan J, Habib M, Kageyama P, Leifert C, Nodari RO, Pengue W. 2010. GM Soy Sustainable? Responsible? GLS Bank, Germany. http://www.global2000.at/module/media/data/global2000.at_de/content/globalnews/
Antoniou M, Habib M, Howard CV, Jennings RC, Leifert C, Nodari RO, Robinson C, Fagan J. 2011. Roundup and Birth Defects: Is the Public Being Kept in the Dark? Earth Open Source. http://www.scribd.com/doc/57277946/RoundupandBirthDefectsv5.
Antoniou M, Habib ME, Howard CV, Jennings RC, Leifert C, Nodari RO, Robinson CJ, Fagan J. 2012. Teratogenic effects of glyphosate-based herbicides: divergence of regulatory decisions from scientific evidence. J Environ Anal Toxicol S:4:006.
Astiz M, de Alaniz MJT, Marra CA. 2009. The impact of simultaneous intoxication with agrochemicals on the antioxidant defense system in rat. Pestic Biochem Physiol 94:93-9.
Barky FA, Abdelsalam HA, Mahmoud MB, Hamdi SA. 2012. Inﬂuence of Atrazine and Roundup pesticides on biochemical and molecular aspects
of Biomphalaria alexandrina snails. Pestic Biochem Physiol 104(1):9-18
Benamu MA, Schneider MI, Sanchez NE. 2010. Effects of the herbicide glyphosate on biological attributes of Alpaida veniliae (Araneae, Araneidae), in laboratory. Chemosphere 78:871-6.
Benítez-Leite S, Macchi M A, Acosta M. 2009. Malformaciones congénitas asociadas a agrotóxicos. Arch Pediatr drug 80:237-47. Cited in Paganelli et al 2010.
Brandli D, Reinacher S. 2012. Herbicides found in human urine. Ithaka Journal Jan 270-272.
Brower LP, Taylor OR, Williams EH, Slayback DA, Zubieta RR, Ramirez MI. 2011. Decline of monarch butterﬂies overwintering in Mexico: is the migratory phenomenon at risk? Insect Conserv Divers 5(2):95-100.
Chaco. 2010. Primer Informe de Comision Provincial de Investigación de Contaminantes del Agua. http://www.gmwatch.eu/files/Chaco_Government_Report_Spanish.pdf.
Chang F-C, Simcik MF, Capel PD. 2011. Occurrence and fate of the herbicide glyphosate and its degradate aminomethylphosphonic acid in the atmosphere.
Environ Toxicol Chem 30(3):548-55.Clair E, Mesnage R, Travert C, Seralini G-E. 2012a. A glyphosate-based herbicide induces necrosis and apoptosis in mature rat testicular cells in vitro, and testosterone decrease at lower levels. Toxicol in Vitro 26:269-79.
Clair E, Linn L, Travert C, Amiel C, Seralini G-E, Panoff J-M. 2012b. Effects of Roundup and glyphosate on three food microorganisms: Geotrichum candidum, Lactococcus lactis subsp. cremoris and Lactobacillus delbrueckii subsp. bulgaricus. Curr Microbiol 64(5):486-91.
Contardo-Jara V, Klingelmann E, Wiegand C. 2009. Bioaccumulation of glyphosate and its formulation Roundup Ultra in Lumbriculus variegatus and its effects on biotransformation and antioxidant enzymes. Environ Pollut 157:57-63.
Dawson AH, Eddleston M, Senarathna L, Mohamed F, Gawarammana I, Bowe SJ, Manuweera G, Buckley NA. 2010. Acute human lethal toxicity of agricultural pesticides: a prospective cohort study. PLoS Med 7(10): e1000357. doi:10.1371/journal.
Druart C, Millet M, Scheiﬂer R, Delhomme O, Raeppel C, de Vaulﬂeury A. 2011. Snails as indicators of pesticide drift, deposit, transfer and effects in the vineyard. Sci Total Environ 409(20):4280-8.
Dutra BK, Fernandes FA, Failace DM, Oliveira GT. 2011. Effect of Roundup (glyphosate formulation) in the energy metabolism and reproductive traits of Hyalella castroi (Crustacea, Amphipoda, Dogielinotidae). Ecotoxicology 20:255-63.
Elie-Caille C, Heu C, Guyon C, Nicod L. 2010. Morphological damages of a glyphosate-treated human keratinocytes cell line revealed by a micro- to nanoscale microscopic investigation. Cell Biol Toxicol 26:331-9.
Filip I. 2012. Monsanto and Big Tobacco Blamed for Birth Defects. Courthouse News Service, Wilmington. http://www.courthousenews.com/2012/04/10/45469.htm.
George J, Prasad S, Mahmood Z, Shukla Y. 2009. Studies on glyphosate-induced carcinogenicity in mouse skin: a proteomic approach. J Proteomics 73:951-64.
Gerislioglu A, Gungormus C, Korkmaz A, Kolankaya D. 2010. Embryotoxic and teratogenic effects of Roundup® Max on rat development. Toxicol Letts 196S(S37-351):P204-005.
Ghanem A, Bados P, Estaun AR, de Alencastro LF, Taibi S, Einhorn J, Mougin C. 2007. Concentrations and specific loads of glyphosate, diuron, atrazine, nonylphenol and metabolites thereof in French urban sewage sludge. Chemosphere 69:1368-73.GM Freeze. 2011. Weed resistance in RR Crops – an Update. October. http://www.gmfreeze.org/site_media/uploads/publications/glyphosate_brief_final.pdf.
Griesinger LM, Evans SC, Rypstra AL. 2011. Effects of a glyphosate-based herbicide on mate location in wolf spider that inhabits agroecosystems. Chemosphere 84:1461-6.
Gui YX, Fan XN, Wang HM, Wang G, Chen SD. 2012. Glyphosate induced cell death through apoptotic and autophagic mechanisms. Neurol Teratol 34:344-9.
Hanke I, Wittmer I, Bischofberger S, Stamm C, Singer H. 2010. Relevance of urban glyphosate use for surface water quality. Chemosphere 81:422-9.
Heu C, Elie-Caille C, Mougey V, Launay S, Nicod L. 2012a. A step further toward glyphosate-induced epidermal cell death: Involvement of mitochondrial and oxidative mechanisms. Environ Toxicol Pharmacol 34:144-53.
Heu C, Berquand A, Elie-Caille C, Nicod L. 2012b. Glyphosate-induced stiffening of HaCaT keratinocytes, a Peak Force Tapping study in living cells. J Struc Biol 178:1-7.
Ho M-W. 2011. Scientist defends claim of new pathogen linked to GM crops. ISIS Report 21/4/11. http://www.i-sis.org.uk/
Kelly DW, Poulin R, Tompkins DM, Townsend CR. 2010. Synergistic effects of glyphosate formulation and parasite infection on fish malformations and survival. J Appld Ecol 47(2):498-504.
Koller VJ, Furhacker M, Nersesyan A, Misik M, Eisenbauer M, Knasmueller S. 2012. Cytotoxic and DNA-damaging properties of glyphosate and Roundup in human-derived buccal epithelial cells. Arch Toxicol 86(5):805-13.
Mamy L, Gabrielle B, Barriuso E. 2010. Comparative environmental impacts of glyphosate and conventional herbicides when used with glyphosate-tolerant and non-tolerant crops. Environ Pollut 158:3172-8.
Manea A, Leishman MR, Downey PO. 2011. Exotic C4 grasses have increased tolerance to glyphosate under elevated carbon dioxide. Weed Sci 59:28-36.
Mann RM, Hyne RV, Choung CB, Wilson SP. 2009. Amphibians and agricultural chemicals: Review of the risks in a complex environment. Environ Pollut 157:2903-27.